The effects of traumatic brain injury persist far longer than just the acute, post-injury period. Individuals suffering TBI often complain of disrupted sleep, which effects cognitive performance and mood, among other symptoms. These symptoms may persist for years or decades. This project tests the hypothesis that replacement of a key brain chemical will alleviate post-TBI sleep disturbances. During the first year of funding from MINDSOURCE, the team collected tissue from 55 experimental mice to determine effects of TBI on the brain cells that contain brain chemical in which they are interested. Approximately 50% of the tissue we collected was processed before research halted in response to COVID-19. They anticipate returning to (limited) research in the very near future, and will continue processing these samples. Once tissue processing is complete, they will have a good assessment of TBI on these types of brain cells, and will then proceed to the next phase of the study.